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I'll perform as directed but no more than that.
Extracellular lactate measurement was performed as directed (BioVision) using a lactate assay kit kindly provided by Prof. Burlina (Department of Paediatrics, University of Padova).
Proteins were electrophoresed on 10% Tris-HCl polyacrylamide gels at 100 V for 60 min and transferred on PVDF membranes at 100 V for 60 min. Blotting was then performed as directed by the antibody manufacturer.
The hybridization procedure was performed as directed by the manufacturer.
Procedures were performed as directed by the manufacturer.
Additional laboratory testing was performed as directed by the intensivist.
The sample preparation, including DNA extraction, digestion, labelling and hybridization, was performed as directed by the manufacturer.
Reactions were performed as directed except using half the recommended reaction volume and one quarter the recommended Cy-CTP amount.
The experiment was performed as directed in the kit manual and 1 μl of the RNA preparation was used for the analysis.
PASA [ 61] assemblies were performed as directed on the software homepage [ 97], with the following modifications: Per default PASA uses GMAP [ 62] for transcript alignments, however, our evaluation process (data not shown) reveals improved EST alignments using GenomeThreader.
Reverse transcription of 10 ng of total RNA with primers corresponding to each miRNA and to U48 small nuclear RNA (RNU48) as endogenous control was performed as directed by the protocol of the two-step TaqMan MicroRNA Assay kit (Applied Biosystems, Foster City, CA, USA) that incorporates a target-specific stem-loop reverse transcription primer to provide specificity for the mature miRNA target.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com