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In this study, we prepared ethyl-acetate extract from the air-dried leaves of Toona microcarpa Harms and investigated the anticoagulant activities in vitro by performing activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) assays.
Firstly, experimental activity has been carried out for identification of the best performing activated carbon available on the market, through the evaluation of the achievable thermodynamic performance both under air conditioning and refrigeration conditions.
Once identified the best performing activated carbon, the design of the adsorber was developed by experimental dynamic performance analysis, carried out by means of the Gravimetric-Large Temperature Jump (G-LTJ) apparatus available at CNR ITAE lab.
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Finally, we performed activated caspase-3 western blot analysis.
The four major MAP Kinase pathways, ERK, JNK, P38, and BMK/ERK5, perform activating phosphorylations of nuclear transcription factors.
Three ROTEM® tests were performed: extrinsically activated assay with tissue factor (EXTEM), intrinsically activated test using kaolin (INTEM), and extrinsically activated test with tissue factor and the platelet inhibitor cytochalasin D (FIBTEM).
We performed extrinsically activated thromboelastometric test (EXTEM), a test that uses rabbit brain thromboplastin as an activator, and fibrin-based thromboelastometric test (FibTEM), a test that assesses the fibrin-based clot using both extrinsic activation and addition of cytochalasin D to inhibit platelets' contribution to the formation of the clot.
We also show over-expression of the CkTLP gene in transgenic Arabidopsis performed or activated resistance against V. dahliae, which affects more than 400 plant types and limits the crop yield especially in cotton [21].
To analyze gene expression in zebrafish leukocytes, we performed fluorescence activated cell sorting (FACS) isolation of EGFP-positive neutrophils from Tg lyzC EGFP) larvae.
In addition to genetic analysis, the immunohistochemical study of the EGFR protein was performed and activated ERK1/2 were assessed by using an antibody specific for the dually phosphorylated and activated ERK1 and ERK2 (MAPK phospho-p44/42 phospho-p44/42
We performed fluorescence activated cell sorting (FACS) of neuronal nuclei and Illumina HM450 DNA methylation profiling in post mortem frontal cortex of 29 major depression and 29 matched controls.
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