In the second part of the experimental analysis, several tests are performed with the combined injection of gasoline and CNG.
While the intra-articular injection of sgp130 was performed simultaneously with the injection of the antigen into the knee joint and can therefore be considered as pre-treatment, systemic sgp130 was administered for the first time six hours after induction of inflammation, that is, as post-treatment (same treatment regime as for etanercept and infliximab).
To eliminate signal from the blood vessels and transparently image the EPR of the probe, contrast-enhanced imaging was performed with IV injection of the probe at a dose of 455 mg/kg b.w.
Precursor and product ion selection as well as the optimization of collision energies were performed with flow injection of single analyte solutions using the MassHunter Optimizer software.
Precursor and product ion selection and the optimisation of fragmentor voltages and collision energies were performed with flow injection of single-analyte solutions using MassHunter Optimizer Triple Quad B04.01.
Under brief isoflurane anaesthesia (5% isoflurane for anaesthesia induction, 2% for maintenance), an intra-articular injection was performed with the use of a Hamilton syringe, with a 26 G needle inserted through the patellar ligament into the joint space of the left knee.
A diagnostic pars plana vitrectomy was performed with intravitreal injection of 5 μg amphotericin.
Premedication was performed with intramuscular injection of ketamine (10 mg kg−1), azaperone (4 mg kg−1), midazolam (0.5 mg kg−1), and atropine sulfate (1 mg).
Induction of general anaesthesia will be performed with intravenous injection of propofol (2 to 3 mg/kg), sufentanil (0.2 to 0.3 μg/kg) and cis-astracurium (0.1 to 0.15 mg/kg).
