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Additionally, time domain simulations are also performed with the inclusion of nonlinear track parameters.
Quality control was performed with the inclusion of positive and negative controls in each assay.
Preplanned sensitivity analyses were performed with the inclusion of observational studies.
All simulations were performed with the inclusion of a hydrodynamic correction as proposed in our previous work.
A multivariate logistic regression analysis was performed with the inclusion of independent variables with p < 0.2 in the univariate analysis.
Aggregation assays were also performed with the inclusion of BrdU (Amersham-GE Healthcare, USA) for 4 h prior to fixation.
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Two sensitivity analyses were performed: one with the inclusion of intervention costs and the other with a 1-year instead of a 2-year time horizon.
Then, meta-regressions were performed with the first year of inclusions and the estimated patients' severity of illness at admission as covariates.
Multiple linear regression analysis was performed with forward inclusion of variables with P < 0.10 in univariate analysis.
Subsequently, multivariate models were performed with stepwise inclusion of significant predictors from clinical parameters, routine laboratory and specific biomarkers.
Since B lymphoblastoid cell lines (B-LCL) share many regulatory elements with CD4+ T cells, we performed meta-analysis with the inclusion of the B-LCL data sets (Kasowski et al., 2013a; McVicker et al., 2013a) to increase confidence in shared allele-specific chromatin expression alleles (e.g., ENTPD1 as shown in Figure 4).
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