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PCR was performed with specific primers (Table 3).
PCR was subsequently performed with specific primers (1st; 5′-ATAAGCTGCGCTGCATGA-3′, 2nd; 5′-CAGGAATGGGATGATGATAAGCT-3′) and adaptortor primer.
Subsequent 5′and 3′ RACE performed with specific non-degenerated primers provided two putative cDNAs of the plant BADH family.
The purpose of this paper is to describe an orthograde endodontic retreatment technique performed with specific mechanical instruments made of nickel-titanium (NiTi).
qRT-PCR was performed with specific primers for each genes.
Kinetic enzyme assays were performed with specific chromogenic substrates (Agrawal 2004).
Lane 1 RT-PCR preformed with specific primers for Cry1Ac toxin; lane 2 RT-PCR performed with specific primers for Cyt1Aa toxin.
This section should include: (a) A concise description of the investigation/procedure performed, with specific mention of any non-standard elements of the investigation, e.g. additional sequences.
Future animal model experiments performed with specific C5aR and NF-κB agonists/antagonists or C5aR-deficient mice might more robustly disclose the significance of these pathways.
Stainings were performed with specific antibodies and fluorescently labeled secondary antibodies.
Polymerase chain reactions were performed with specific primers using the Taq polymerase kit (Eurobio).
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