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Statistical analyses of newly acquired data from Sites C0011 and C0012 were performed using the same element suite as that applied to Site 1149.
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These assays were performed using the same primer pairs utilized to determine element copy number abundance via quantitative PCR (see Materials and Methods).
Elution was performed using the same buffer.
Modeling was performed using the same sections.
Lung ultrasound was performed using the same transducer.
Angular measurements were performed using the same software.
All band comparisons were performed using the same selection rectangle.
The operations were performed using the same intraoperative protocol.
First, the ROC analysis was performed using the same sample.
All measurements were performed using the same instrument settings.
PCRs were performed using the same procedure described above.
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