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A simulation of infrared (IR) properties was performed using the same approach to support the proposed mechanism via a complete explanation of bond forming and breaking during each reaction step.
Evolutionary conservation analysis for certain mtDNA variant was performed using the same approach as described in our previous study [15], [25].
Two other rounds of panning, with 10 and 20 washes, were then performed using the same approach.
EWAS in the 15 MZ pairs was performed using the same approach as the analyses in the 41 MZ twin pairs, and the top-ranked results included signals in the promoters (within 200 bp of the TSS) of genes COX7C and U2AF1 that have been previously linked to cancer (Table provided in Additional file 4).
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Finally, for the MBC validation dataset, the detection of stable subgroups was performed using the same unsupervised approach used for our dataset, and these subgroups were then compared with the subgroups from the centroid classification.
The comparative genomics analysis performed using the same methodological approach as applied for true protein-coding genes (see previous section) allows to investigate differences in TE content and distribution.
Analyses of the haplotypes were performed using the same linear regression analysis approach as for the single-marker association analysis.
Parenchymal transection is performed using the same instrumentation as for the open approach, although in our experience ultrasound shears are easier to use laparoscopically.
Targeted metabolomic analysis was performed using the same chromatographic and spectrometric method as untargeted approach.
Targeted lipidomic analysis was performed using the same chromatographic and spectrometric method as untargeted approach.
Further validation of this approach came from repeated experiments performed using the same 454 platform, but different chemistry (Titanium) and different amplicon generation primers.
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CEO of Professional Science Editing for Scientists @ prosciediting.com