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One-step growth curves were performed using a variation of a standard protocol [ 39].
Whole-mount in situ hybridization was performed using a variation of the protocol described by Tautz and Pfeifle [ 40].
Library construction of polyadenylated cDNA was performed using a variation of the Clontech SMART system, in which the 5' and 3' PCR adapters contain type IIs restriction enzyme sites (MmeI).
Adhesion assays were performed using a variation of the protocol described by Scott et al (2001): 4 × 10 serum starved (20 h) PC3-GFP cells were added to a confluent BMEC layer with or without AA (10 μℳ).
Adhesion assays with DU-145 were performed using a variation of Scott et al (2001): 4 × 10 serum-starved (20 h) DU-145 cells were added to a confluent bone marrow endothelial cell (BMEC) layer with or without AA (10 μ M).
Similar(54)
As the differences between 7302R and those none-IPA type lines may reflect the genetic improvement of the IPA-type rice from the current none-IPA cultivars, an investigation of the 7302R-specific variations was therefore performed using a synteny analysis of all the SNPs of the 7302R compared with those of IR24, MH63, and SH527.
Co-variation analyses were performed using a recent implementation of the method of Carlson et al. [4].
For a search performed using a disease name, MitoInteractome provides detailed disease information with associated variations.
Quality control was performed using an internal laboratory standard; the inter-day coefficient of variation (CV) was 2%.
Library preparation was performed by BGI (Hong Kong) using a variation of the Illumina's standard protocol.
Intermittent sonication was performed in 0.5 ml thin-walled PCR tubes using a Misonix 3000MPD device, using a variation of the semi-automated procedure originally described [50].
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