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Plasma extraction was performed using a validated method developed in our laboratory.
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Detection of anti-aflibercept antibodies was performed using a validated quasi-quantitative ELISA method.
Analysis of L-Tryp and L-Kyn was performed using a validated high-performance liquid chromatography with tandem mass spectrometry method as previously described.
Simulated experiments were performed using a validated Monte Carlo model of the MLI to estimate energy absorption and energy separation between the MLI components.
Analysis of anti-prGCD antibodies was performed using a validated immunoassay we have developed (Midwest BioResearch, LLC, Skokie, Illinois).
Assessment of HAHA responses was performed using a validated semiquantitative ELISA.
Screening is performed using a validated automated BP device, the BpTRU (VSM MedTech Ltd ,Coquitlam, Canada).
Pharmacokinetic analysis was performed using a validated enzyme-linked immunosorbent assay (Quantikine® Human G-CSF; R&D Systems GmbH, Wiesbaden-Nordenstadt, Germany).
The miRNA microarray analysis was performed using a validated custom microarray platform.
KIR2DS3 and KIR2DS5 genotyping was performed using a validated PCR, using sequence-specific primers (PCR-SSP) approach [ 40].
Detection of HSV DNA was performed using a well-validated collection and detection method (Magaret et al., 2007).
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