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The study was performed using a spectrofluorometric method, which allows continuous monitoring of the uptake and efflux of fluorescent molecules by living cells.
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Reactions were prepared in 96-well plates and performed using a spectrofluorometric thermal cycler (Biorad iCycler; Hercules, CA).
A flow cytometry assay was performed using a published method.
Kinetic characterization of AChE was performed using a reported method.
Creatinine concentration assessment was performed using a colorimetric method.
Pharmacokinetic analyses were performed using a noncompartmental method.
Quantification of urinary VMA was performed using a liquid chromatographic method (Gironi et al, 1988).
Quantitative data were obtained using Agilent Feature Extraction software (Agilent Technologies) and corrections for background signal intensity for the data were performed using a method, previously described45.
Immunohistochemistry using immunoperoxidase was performed using a free-floating method.
Immunofluorescence was also performed using a free-floating method.
Immunodetection was performed using a standard SABC method.
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