Densitometric quantification analysis of the immunoblot bands was performed using a Molecular Imager FX with Quantity One software (Bio Rad).
Immunoreactive proteins were visualised by enhanced chemiluminescence and densitometric analysis of immunoblots was performed using a Bio-Rad scanning densitometer and Quantity One analyzing software.
Quantitation of PrPres glycoforms was performed using a GS-800 calibrated densitometer and dedicated Quantity One software Bio-Rad Laboratoriess) as described [8].
Densitometry analysis was performed using a GS-700 scanner (Bio-Rad Laboratories) and Quantity One software (Bio-Rad Laboratories).
Densitometric analysis of band intensity was performed using a Biorad Gel Doc XR imaging system and Quantity One 1D analysis software.
Intensity measurement was performed using a BioRad densitometer (GS 800) equipped with Quantity One Software.
To allow precise judgments regarding increases or decreases in each of these quantities, hypothesis testing of the equality of means was performed using a one-tailed test.
Quantification was performed using a CCD camera (ChemiDoc XRS -BioRad) and image analyzer (quantity one - BioRad).
Densitometry was performed using a BIO-RAD GS-710 calibrated imaging densitometer, and Quantity One analysis software.
UV vis spectroscopy was performed using a Biomate 5 Thermo Spectronic spectrometer or Thermo Scientific NanoDrop 2000 (for small quantities).
