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Adhesion assays were performed using a quantitative method based on a previously described fluorescence measurement system [78].
Every fourth stained section, i.e. every 60th section of the series of sections, was examined, resulting in a distance of 1.2 mm between the analyzed sections (Fig. 2).> Cytoarchitectonical analysis was performed using a quantitative method for observer independent and statistically testable detection of cortical borders (Zilles et al. 2002; Schleicher et al. 2005, 2009).
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Scoring of slides was performed using a semi-quantitative method, as reported previously [ 17].
RF quantification was performed using a fully automated, quantitative method to avoid bias, particularly due to the difficulties in delimiting the RFs of many cells within the LPZs (Waleszczyk et al. 2003).
Quantitative urine culture was performed using a dipslide method; urine was also streaked onto MacConkey agar.
Measurement of MMP-9 and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) mRNA was performed using a multiplex quantitative real-time reverse transcriptase PCR method, using primers and probes as previously described (Cioppi et al, 2004).
Quantitative urine culture was performed using a dip-slide method; urine was also streaked on MacConkey agar.
Quantitative bacterial cultures of tibial tissue were performed using a CFU counting method.
Quantitative data were obtained using Agilent Feature Extraction software (Agilent Technologies) and corrections for background signal intensity for the data were performed using a method, previously described45.
Detection of anti-aflibercept antibodies was performed using a validated quasi-quantitative ELISA method.
Quantitative viable bacterial cultures were performed using a colony-forming units (CFU) counting method.
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