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Originally PEPT was performed using a pair of multiwire detectors, and more recently using a pair of digital gamma camera heads.
To investigate the behavior of different types of electrodes, several titrations were performed using a pair of each of platinum, gold and silver as an indicating system.
Semi-quantitative RT-PCR was performed using a pair of specific primers for Otud-6b or Otud-6a (Table S3) at 94°C for 1 min, 50°C for 30 s, and 72°C for 1 min for a total of 35 cycles.
ELISAs were performed using a pair of monoclonal antibodies from BD Pharmingen™, (BD Biosciences).
Semi-quantitative RT-PCR was performed using a pair of gene-specific primers.
Diffusion encoding was performed using a pair of half-sine gradient pulses (δ = 3.2 ms/Δ = 8.3 ms), using b = 800 s/mm.
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Statistical analysis was performed using a paired t test, with the level of significance set at P = 0.05 (SPSS software, version 16.0; SPSS, Chicago, IL).
A comparison of the clonogenic assay of each pair was performed using a paired t-test.
In addition, RT-PCR reactions were performed using a primer pair that anneals to the 5' end of the mRNA and to the 3' end of coding region.
To confirm the gene expression of N2 in the H3N2-VLP producer cell line, total RNAs were extracted separately from cells with and without doxycycline (Dox) induction and RT-PCR assays were performed using a primer pair corresponding to the internal sequence of the N2 gene.
Nucleic acids were extracted from fresh frozen tumour and normal tissue pairs, and whole genome and exome sequencing was performed using a combination of long mate pair and paired-end approaches.
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