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The bioassay was performed using a modified seedling bulk test following the SSST (Velusamy et al. 1986; Horgan 2009).
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Miniprep scale DNA extraction was performed using a modified CTAB protocol by Sambrook and Russell (2001).
For cytokine detection restimulation was performed using a modified protocol.
LCM was performed using a modified protocol for human pancreatic tissue dehydration [18], [19].
Extraction was performed using a modified Bligh and Dyer extraction protocol, optimized for steatotic liver material.
The in vitro migration assay was performed using a modified Boyden chamber [33].
The following analysis steps were performed using a modified version of the AMDA library [68].
Invasion assays were performed using a modified Boyden-chamber assay as described previously [36], [37].
Dot-plot analysis was performed using a modified version of the Java applet "Nucleic Acid Dot Plot" [22].
The extraction of intracellular metabolites from cell pellets was performed using a modified Bligh-Dyer procedure [32].
The validated TNA was performed using a modified version of the protocol provided by Conrad Quinn, CDC Atlanta, GA [16].
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