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The total phenolic content assay was performed using a modified form of the procedure described by Singleton and Rossi (1965).
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Miniprep scale DNA extraction was performed using a modified CTAB protocol by Sambrook and Russell (2001).
For cytokine detection restimulation was performed using a modified protocol.
Invasion assays were performed using a modified Boyden-chamber assay as described previously [36], [37].
The following analysis steps were performed using a modified version of the AMDA library [68].
LCM was performed using a modified protocol for human pancreatic tissue dehydration [18], [19].
Extraction was performed using a modified Bligh and Dyer extraction protocol, optimized for steatotic liver material.
The in vitro migration assay was performed using a modified Boyden chamber [33].
Dose escalation was performed using a modified accelerated-titration design.
Insulin infusion was performed using a modified Yale protocol.
Comparisons were performed using a modified t-test [ 26].
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