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Lipid extraction was performed using a modification of the method described by (Folch et al. 1957).
Transformation was performed using a modification of the previously published Agrobacterium-mediated transformation method (Nishimura et al. 2006).
Covalent attachment of sGNRs to the MWNTs was performed using a modification of the standard EDC/NHS reaction[49, 50].
Gelatinase zymography was performed using a modification of Kherif et al [44].
Immunoblotting was performed using a modification of the method of Besch et al [26].
SSH was performed using a modification of the protocol given by Diatchenko et al. [17].
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Probe preparation and in situ hybridization were performed using a modification [ 135] of the protocol of Ferrándiz et al. [ 136].
DNA was sheared by sonication and Western blotting analysis was performed using a slight modification of the method first described by Towbin et al. [ 19].
The antibody staining protocols used to assess proliferation (1/800 Phospho-Histone H3, Cell Signaling, 9701) and apoptosis (1/200 Cleaved Caspase-3, Cell Signaling, 9661) were performed using a minor modification of a previously reported method (25).
Vessel segmentation was performed using a custom made modification of the Seeded Region Growing algorithm that was designed in order to identify pixels belonging to the breast vascular network.
Bisulfite treatment of genomic DNA was performed using a CpGenome DNA modification kit (INTERGEN).
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