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Stimulation was performed using a mixture of lipopolysacchride (LPS), peptidoglycan (PGN), and zymosan (ZYM).
Segmentation was performed using a mixture of an automated approach with manual localized visual refinements to the tooth structure.
The functionalization was performed using a mixture of non-organic acids producing COOH functional groups of 1.23 wt% of the MWCNTs weight.
A test of the chip for a two-label fluorescence case was performed using a mixture of quantum dots in a priori unknown proportions.
Therefore, according to the aim of the researcher, the change detection analysis can be performed using a mixture of more techniques, the so-called hybrid approach, that combines two or more techniques in order to improve the results [6, 7].
The benzyne functionalization of chemical vapor deposition grown large area graphene and graphite was performed using a mixture of o-trimethylsilylphenyl triflate and cesium fluoride that react with the carbon surface.
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Cleavage from the resin (2 × 30 min) and concomitant t Bu-deprotection was performed using a mixture (v/v/v) of 95% TFA, 2.5% triisobutylsilane (TIBS) and 2.5% water.
Because PAI-1 displays high affinity and specificity for the SMTB 1 44 domain of VTNC [15] [20] competition experiments were performed using a mixture containing Cyr61 and increasing concentrations of stable mutant PAI-1.
Kinase assays using purified CDK complexes were performed using a reaction mixture of 0.2 ug kinase complex or PKA, 1 ul of 100 mM ATP, and 0.5 ug GST fusion protein diluted to a final volume of 35 ul with kinase buffer.
Chromatography was performed using a solvent mixture of ethyl acetate:methanol:25% ammoniumhydroxide solution at a volume ratio of 37.5 12.5 1.
HPLC analysis was performed using a 95∶5 mixture of solvents A (0.1% formic acid) and B (0.08% formic acid in methanol), respectively.
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