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The measuring procedure begins with the calibration which is performed using a material of the known thermal conductivity and typical specimen dimensions.
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Mechanical evaluation was performed using a computerized materials testing machine (100R; DDL Inc., Eden Prarie, MN).
Identification of BFo-like colonies was performed using a combinatorial approach of 16S colony PCR and reconstruction of an NJ phylogeny (supplementary fig. S1, Supplementary Material online).
In many cases, after removal of the cyst, bone grafting is performed using a particle-type bone graft material.
Ultrafast photoexcitation of GaAs material was performed using a mode-locked Ti/Sapphire laser as an excitation source, centered at 850 nm wavelength, emitting 100 fs pulses at a repetition rate of 80 MHz.
Injections were performed using a patch-clamp apparatus (see Materials and Methods).
AFM imaging of the two nanocellulose materials was performed using a Dimension 3100 AFM (Bruker) and RTESPA probes (Bruker).
Visualisation was performed using a widefield microscope (Figure 1C; see Materials and Methods; Figure 2A D).
The reactions were performed using a thermocycler iCycler IQ (BioRad; Hercules, CA), using 2 μg of RNA as starting material.
Extraction was performed using a modified Bligh and Dyer extraction protocol, optimized for steatotic liver material.
Confocal microscopy was employed for verification of encapsulation of fluorescent material, and was performed using an Olympus FV1000 confocal microscope.
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