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The quantification of intracellular adenine nucleotides was performed using a fluorometric method as described.
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The glucosylceramidase enzymatic assay was performed using a fluorometric assay with 10 mM 4-methylumbelliferyl-β d-glucopyranoside (Sigma) as the substrate.
TUNEL analysis was performed using a Deadend Fluorometric TUNEL System (Promega, Poland).
The TUNEL assay was performed using a DeadEnd Fluorometric TUNEL System (Promega) according to the manufacturer's instructions.
TUNEL analysis was performed using a Deadend Fluorometric TUNEL System, (Promega, Poland) according to the protocol described in Warzych et al. [ 19].
Each stage of the impactor was calibrated using a gravimetric or counting method and the fully assembled impactor was evaluated by using a fluorometric method.
We recommend using a fluorometric method, for example, with PicoGreen or the Qubit system (Cat Number Q32866, Life Technologies, USA).
Total intracellular TG content was measured using a fluorometric method kit (BioVision, CA, USA) in accordance with the manufacturer's instructions.
Plasma or tissue homogenates were deproteinated and nitrite content was then quantified using a fluorometric method utilizing 2,3-diaminonaphthalene (Bryan and Grisham, 2007).
TUNEL staining was performed using DeadEnd fluorometric TUNEL system (Promega) following the manufacturer's directions.
TUNEL analysis was performed using DeadEnd™ Fluorometric TUNEL System kit (Promega).
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