Sentence examples for performed under denaturing from inspiring English sources

The correctness of the amino acid sequences of the recombinant allergens was verified by ESI-MS measurements of the monomeric proteins, performed under denaturing solution conditions (Table S1).

The zymogram analyses of purified Spr Cel8A, performed under denaturing conditions, showed a single band that confirmed the cellulolytic activity and monomeric nature of the enzyme (Fig. 2c).

However, when the purification was performed under denaturing conditions, high yields of pure YY1 were readily produced (Fig. 1A).

One-dimensional SDS-PAGE was performed under denaturing and reducing conditions, transferred onto nitrocellulose membranes (Schleicher & Schüll, Dassel, Germany), which were blocked overnight at 4°C with 5% non-fat milk in Tris Buffered Saline-Tween 20 (TBS-T).

HA immunoprecipitations were performed under denaturing conditions.

The purification was performed under denaturing conditions with FastFlow Sepharose (GE Healthcare) loaded with nickel.

Western blot analysis was performed under standard denaturing conditions using following antibodies at 1 : 1000 dilutions: cleaved caspase-3 (Cell Signalling Technology CSTT), Denver, MA, USA), BCL2 (Cell Signalling Technology), α-tubulin (Sigma), GAPDH (Abmart, Arlington, MA, USA), AKT Cell Signalling Technologyy), AKT3 (Cell Signalling Technology and GFPP (Roche, Indianapolis, IN, USA).

The reaction was performed under the following conditions: denaturing at 94°C for 1 min, followed by 30 cycles of denaturing at 98°C for 10 s and elongating at 70°C for 4 min (without additional primer annealing step), and finally, an extended elongation period of 10 min at 72°C.

PCR was performed under the following conditions: denaturing at 95°C for 20 s followed by 40 cycles of 95°C for 1 s and 60°C for 20 s.

WB analyses were performed on sodium dodecyl sulfate polyacrylamide gel electrophoresis gels under denaturing conditions as described previously using 50 µg of whole cell lysate per lane, except for α6A and α6B immunodetections, which were performed under non-denaturing conditions using 120 µg of whole cell lysate per lane (12, 31).

The RT-PCR was performed under the following steps: initial denaturing step at 94 °C for 5 min, 35 thermal cycling step consisted of 30 s at 94 °C, 30 s at 56 °C and 30 s at 72 °C, and then final extension step at 72 °C for 5 min for termination.