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Eight days after fertilisation the blastocyst had started to hatch and mechanical isolation was performed to isolate the ICM.
Mechanical isolation was performed to isolate the spindle-shaped (SS) AF-MSCs, according to methods described in our previous studies.
Subsequently, sensitivity analyses are performed to isolate the impacts of false detections and long-term, magnitude-dependent bias in CMORPH on the inaccuracies in FC and correlograms.
Because the GFP gene is randomly inactivated in the MEFs, fluorescence activated cell sorting (FACS) was performed to isolate only GFP-negative MEFs that carry the GFP transgene on an inactive X chromosome, prior to reprogramming.
Supercritical fluid extraction with carbon dioxide (SC-CO2 extraction) was performed to isolate essential oils from the rhizomes of Cyperus rotundus Linn.
A concept named time to surge is introduced and a sensitivity analysis is performed to isolate the important characteristics that influence surge transients in an engine.
In a previous study, activated platelets or αIIbβ3-expressing CHO cells selection was performed to isolate human IgG anti-αIIbβ3 binding fragments using combinatorial libraries created from the B cells of a GT and an AITP patient.
Therefore, further work must be performed to isolate and identify these components.
Further, traditional mutagenesis with intelligent screening/selection design was performed to isolate hyper-cellulolytic mutants.
This procedure was performed to isolate rifampicin-resistant mutants (Rif +) of EB and was previously described by Kloepper (1980).
Using the optimal staining conditions determined above, ASP+-guided FACS was performed to isolate hNET positive SupT1 cells by gating on the brightest 5% of each population.
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