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Quantum mechanical ab initio simulations were performed to complement the experiments.
The numerical finite element (FE) analysis is also performed to complement the damage detection.
Moreover, the DFT calculations were performed to complement the experimental evidences.
Color segmentation of the active and static areas of each video frame is performed to complement this information.
Also, steered MD simulations are performed to complement experiments.
Sanger sequencing was performed to complement the FLX run data with its long sequence reads.
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The locus at which the wild type gene is reintroduced and unspecific effects due to more rounds of genetic manipulation performed to construct complemented strains might also affect virulence in this model.
Hemolytic assays (e.g., CH50 or AH50) are widely performed to assess complement activity and detect complement deficiency, but there are some problems in terms of cost and effectiveness.
Functional studies were performed to evaluate complement resistance of tumour cells after shRNA-mediated knock-down of DAF and/or MCP expression.
Real-time PCR assays were also performed to confirm and complement MLPA results.
Meanwhile, parallel experiments of targeting the same specific genes in human ESCs could also be performed to control and to complement the iPSC-based approach for ALS disease modeling studies.
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