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After contact durations of 15, 30, 45, 60, 90, 120, and 180 min, sampling was performed; the samples were centrifuged at a speed of 6000 rpm during 30 min.
Based on the plateaus in ac conductivity, the dc conductivity was computed, and found almost 400%% increase in electrical conductivity for 20 wt.% at 278.15 K. JF planned the experiments, performed the samples, conducted the studies, has made the processing and analysis of data, took an active part in the discussion of the results and preparation of the manuscript.
When sequential extraction was performed the samples were first extracted with detergent free RAB buffer (MES 100 mM, EGTA 1 mM, MgSO4 0.5 mM, NaCl 750 mM, NaF 20 mM, EDTA 100 mM, Na2VO4 1 mM, PMSF 1 mM, pH 6.5) containing 2x Complete proteinase inhibitor (Roche Diagnostics, Mannheim, Germany) followed by a RIPA extraction of the pellet.
If a biopsy is performed, the samples should include the fibrous scar, if present, because diagnostic bile ductules may be found only in this region of the tumor[ 53 ].
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AL performed the sample analysis.
XB performed the sample preparation.
VD, BO, and SA performed the sample preparation and characterization.
AA and VH performed the sampling and analyses.
BKC, IHL, and JK performed the sample characterizations.
JA and JQ performed the sample fabrication and characterization.
KI performed the sample preparation, discussed the results and drafted the manuscript.
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CEO of Professional Science Editing for Scientists @ prosciediting.com