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In order to verify that the Gas6-dependent decrease of migration was due to specific ligation and activation of the Axl receptor by Gas6 we performed the migration experiment using Axl knockdown cells.
ZS and RM performed the migration assay.
HQY performed the migration, invasion and gene expression experiments.
AP and AJ designed and performed the migration, tube formation and immunofluorescence experiments.
HEC also performed the migration, proliferation and transfection experiments with the assistance of TW, DLD and PD.
We performed the migration assay without the use of Matrigel, however, there was no up-regulation of NO-induced migration activity (data not shown).
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This reflects how some of our approach users can perform the migration with the system shutdown.
Finally, the execution (E) component performs the migration of the VMs in addition to switching the unused PMs off.
This supports migrations without middleware (e.g., if database replication functionality is performing the migration) or those where a VPN is not required.
For blocking studies, both the lower well containing CCL21 (100 ng/ml) and the upper well containing the DCs were pre-treated with 10 µg/ml galactose or α2,8-linked polysialic acid coupled to Polyacrylamide (Lectinity) for 45 min. at 37°C before performing the migration assay.
The addition of the C-terminal hydrophobic tail made the peptide further insoluble and, therefore, hampered the ability to perform the migration assay at higher doses (i.e. over several μM).
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CEO of Professional Science Editing for Scientists @ prosciediting.com