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RK and TK performed the confirmation experiments.
We performed the confirmation of EGR2, RGS1, FOXO3A, HIF1A, IL1B and SOD2 genes, which were chosen because some of these genes have been previously described as specific for GDM compared with T2D (SOD2, FOXO3A, HIF1A).
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The predicted optimum results have been verified by performing the confirmation tests.
Further, the predicted models have been verified by performing the confirmation experiments.
Finally, optimum level of process parameters that simultaneously minimizes the deviation in both the dimensions have been found out using response optimization module of Minitab 17 software and the results obtained have been verified by performing the confirmation experiments.
SL prepared experiment samples, performed the experiment confirmation of transcriptome structure, participated in data analysis and manuscript revision.
To validate the microarray analysis we also performed the PCR confirmation step on all genes identified as essential by the automated method, and also on all genes not identified as essential in S. aureus, but which had an essential orthologue in B. subtilis [ 4].
JNA performed the scrapie status confirmation and the strain determination.
NK performed the PCR for confirmation of operon structure and qRT-PCR experiments.
PCR amplification using the same DNA primers as abovementioned was further performed for the confirmation of the JM83-AAT24, JM83-ATT24.
Even though Western blot was performed for the confirmation of the ELISA results antibodies of leptospires were measured to rule out cross reaction in the ELISA.
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