D.d.R. performed testes-staging analysis.
LG performed the testes RNA preparations and RT-PCR for the different Drosophila species.
Finally, immunofluorescence for β-galactosidase was performed on testes from SAFB2+/+ and SAFB2−/− mice, indicating the presence of β-galactosidase in SAFB2−/− Sertoli cells, and also in the germ cells although at a lower level (Fig. 1F).
Single colorimetric immunohistochemistry was also performed on d100 testes for WT-1 to demonstrate the location of SC nuclei and for 3β-HSD to highlight LCs [8].
IHC was also performed on mouse testes at 7, 15, 30 and 40 dpp.
Comparison of gene expression between groups of tumor was performed by nonparametric testes Mann-Whitney and Kruskall-Wallis.
Nevertheless, in the light of the obtained P-values and the number of statistical testes performed, we cannot exclude that our positive findings may be due to chance.
Laboratory testes performed to rule out pituitary or parathyroid tumors were serum fasting prolactin, ACTH, cortisol, growth hormone and insulin like growth factor1, T4, TSH, intact PTH, calcium and phosphorus.
Heterozygous and homozygous variant genotype carriers were combined for the analyses of interactions due to power-considerations. Therefore, in the light of the obtained P-values and the number of statistical testes performed, we cannot exclude that our positive findings are due to chance.
Also, histopathological examinations of the testes were performed.
Scanning of both testes is performed in sagittal and transverse planes with size measurements.
