Exact(2)
To test this possibility, we performed S pulse-chase analysis to measure the rate of degradation of WT Puma and S10A in Bax/Bak DKO MEFs.
We initially performed S methionine uptake assays to determine the concentrations and duration of CHX treatment required to inhibit protein synthesis in both mammary epithelial and breast cancer cells.
Similar(58)
To test this, we performed S-beads pull-down of TRIM24-SFB in 293T cells that were co-transfected with TRIM24-SFB and HA-Ubiquitin.
Next, to investigate whether this is due to a disruption of SPOP binding to TRIM24, we performed S-beads pull-down of TRIM24 in 293T cells co-transfected with TRIM24-SFB, Myc-SPOP, and/or HA-TRIM28.
That would argue for s-all to be the primary non-parametric method but for comparison we also performed s-pairs analyses.
ELV participated in the animal experiments, carried out histological processing of the knee joints, participated in immunohistochemistry and performed S-sulfate measurements.
To directly test that protein translation is indeed affected by cohesin depletion, we performed S-methionine metabolic labeling to measure protein biosynthesis and H-uridine labeling to measure rRNA production in the cohesin morphants with and without l-Leu supplement at 1 dpf (Fig. 4).
A Carol for Our Times: The Melrose Quartet perform "Sing John Ball" from Michal Shapiro on Vimeo.
All analyses were performed using S Plus.
Analysis performed with 0.5 s segments (c, d).
Analysis was performed on 10-s records.
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