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In each case, a standard scan was performed ranging from 300 to 1000 nm in increments of 10 nm for the 65°, 70° and 75° incident angles.
With this freely available software, simulations can be performed ranging from microscopic classical molecular dynamics up to the Lagrangian particle-based discretisation of macroscopic continuum mechanics equations.
Two parallel MS2 scans were performed ranging from 120 800 a.m.u., using 15 and 45 V cone voltages.
To verify the specificity of each primer pair, a melting curve analysis was performed ranging from 60°C to 95°C with temperature increasing steps of 0.06°C/s (5 acquisitions per °C) at the end of each run.
It was also shown that the risk of perforations depends on the type of surgery performed, ranging from 7% in urological surgery up to 65% in cardiac surgery [ 4- 8].
To confirm specificity of primer pairs and establish melting temperatures (Tm) a temperature gradient was performed ranging from 57.0°C – 65.3°C using a 4 times dilution series of cDNA from blastocyst samples.
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The superficial gas velocities at which laser-based planar imaging experiments were performed ranged from about 4 8 times the minimum fluidization velocity.
The amount of IR recession performed ranged from 5 mm to 7 mm (median 6.5 mm).
The amount of MR recession performed ranged from 4 mm to 11.5 mm (median = 7.5 mm).
Because there was some temperature variation from run to run and during the runs, the temperature at which the treatments were performed ranged from 150 to 160 °C.
The number of amplification cycles performed ranged from 28 to 33, in order to maintain the semi-quantitative nature of PCR but minimise non-specific amplification.
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