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The hydroxylation was performed overnight by dispersing the ground powder in 80 mL NaOH solution.
First, a Thellier measurement that is very laborious and time-consuming can be performed overnight without any user intervention.
Following the epMEGAWHOP PCR, Dpn I digestion (20 U) of the template was performed overnight at 37°C.
Any heavy maintenance is performed overnight.
Rehybridization was performed overnight at 65°C.
Immunoprecipitation and DNA recovery were performed overnight.
Hybridizations were performed overnight at 42°C.
Immunoprecipitations were performed overnight at 4°C.
Incubation with specific antibodies was performed overnight at 4°C.
Immersion fixation was performed overnight in the same fixative.
Immunoprecipitation was performed overnight with antibodies against C/EBP α.
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