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Chromosomal analysis (G-banding) was performed on preparations from 48-h bone marrow cell cultures where mitogens were not added, according to a protocol from the American Type Culture Collection.
Three reviews (covering a total of about 30 trials) have been performed on preparations containing extracts of Echinacea (Echinacea purpurea, pallida or angustifolia), two of which by the same study group [ 40- 43].
A preliminary account of some of this work has previously been published in an abstract form [21]. Experiments were performed on preparations of the longitudinal muscle layer of the guinea-pig ileum: (1) freshly isolated SMCs or (2) freshly dissected strips of smooth muscles (see below).
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Fluorescent in-situ hybridisation was performed on touch preparation samples prepared from fresh endoscopic biopsies obtained from the enterovesical anastomosis and native bladder remnant (control specimens) of 15 patients who had undergone a clam ileocystoplasty.
CYP activity was performed on liver preparations according to the procedure described in the kit from the (Enzo Life Sciences, Inc., UK).
The bioassays were performed on microbial preparations obtained after either direct dilution of soil suspensions or prior density-gradient separation of microbial cells from the soil matrix.
Multicolour FISH was subsequently performed on interphase preparations originating from patients with Barrett's esophagus at varying histological grades, thus demonstrating the whole region of chromosome 4 was amplified within the tissues.
Red blood cell lysis was performed on splenocyte preparations.
Differential cell counts were performed on cytocentrifuge preparations stained with hematoxylin and eosin.
For determination of the breakpoint on chromosome 1 FISH was performed on metaphase preparations of the cell line S40.2.
Quantification of bacterial CFU was performed on probiotic preparations by plate count method, and mouse feces were checked daily for the presence of the transgenic Lactobacillus.
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