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Experiments were performed in replicates of at least six wells.
Experiments in n = 3 wells were performed in replicates.
All tests, and their respective control samples, were performed in replicates of three.
Fourteen-day colony formation assays were conducted as described by us39, using human cord blood CD34+ stem cells (AllCells Inc ., using MethoCultExpress™ colony growth media (StemCell Technologies Inc ., performed in replicates of N = 2.
All tests, and their respective control samples, were performed in replicates of 3. Checkerboard assays37, 38 were performed in 96-well polystyrene plates, in order to unveil the synergistic effects of silver and transition metals.
In LD50 testing, several dose levels of the test compound are used, with one dose per group of animals, and the testing is performed in replicates.
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All experiments were performed in replicate independently.
Experiments to test quenching efficiency were performed in replicate using Escherichia coli lysate.
Experiments were performed in replicate.
The procedure was performed in replicate.
All reactions were performed in replicate (duplicate or triplicate) in 96-well plates.
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