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Densitometry was performed for quantification.
There are three essential steps, which have to be performed for quantification of SPECT images.
Subsequently, Northern analysis was performed for quantification of specific gene dynamics and statistically significant overlap in the regulation of 11 stress-related genes was found using correlation analysis.
Gas chromatography-flame ionization analysis was performed for quantification of cholesterol and mass spectrometry was performed for lathosterol and plant sterols as described previously [22].
Multiple Reaction Monitoring (MRM) mode was performed for quantification.
Three independent experiments were performed for quantification of the Sec body phenotype as scored by immunofluorescence.
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A novel strategy using the absorbance ratio of transverse and longitudinal LSPR peak of GNRs as a function of AFP contraction was performed for AFP quantification.
In selected cases, real-time PCR was also performed for mRNA quantification.
Seven days after choroid lesions appeared at thirty different locations, total RNA extraction was performed for miRNA quantification as described above.
Immunohistochemical analysis of muscle cross-sections was performed for the quantification of SCs (Pax7+ cells) as well as the expression of phosphorylated STAT3, IL-6, IL-6Rα, and PCNA across all time-points.
Three independent qPCR reactions were performed for final quantification.
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