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EG and GR performed domain analyses.
We then performed domain analysis on all hits as described below in section II.
In particular, we performed domain analysis for the 5 17 years age group [ 39].
DK collected all sequence data, performed domain mapping, multiple sequence alignment, phylogenetic tree analysis, and mined published gene expression data.
Therefore, we performed domain search by using the Pfam and the NCBI Conserved Domain database, which revealed a wide range of functionally important domains in the fly DUBs.
To identify the region within TDP-43 that is required for localization to Gems, we performed domain analysis using deletion mutants of TDP-43 (Fig 2A).
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By using known invertebrate Tectonin proteins, we performed domain- and conserved position-specific iterated sequence searches, and identified a potential human homolog, which we dubbed the hTectonin.
We aligned the seven sequences of genomic segments containing 2DL5A or 2DL5B and the neighboring 2DS3 or 2DS5 (Figure 5A), and performed domain-by-domain phylogenetic analysis with construction of neighbor-joining and parsimony trees.
To investigate further the observation that 3DL3 is highly polymorphic in both Cen-A and Cen-B, we performed domain-by-domain phylogenetic analysis of the alleles of this gene (Figure 4).
First, to gain insight into the apparent phosphopeptide binding activity of human PIH1D1 and its interactions with R2TP complex components, we performed domain-mapping experiments.
With these sets, we performed domain-centered sequence alignments using ClustalW and Omega (Sievers et al., 2011) followed by subsequent manual refinement.
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