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In parallel, we performed degradation assays in which we incubated in vitro synthesized 35S-labeled proteins with an HEK293 extract harvested from cells exiting mitosis.
We performed degradation by heat-treatment of isolated RNA to avoid transcriptional processes in the context of cell death.
We performed degradation of MBP by cerebral proteasomes from BALB/c mice in water containing oxygen with mass 18. Proteasomes from the brains of intact SJL and EAE-SJL mice hydrolyzed MBP in ordinary water.
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FReET-D software is capable of performing degradation modeling, in which a large number of reinforced concrete degradation models can be utilized under the main FReET software engine.
We first present a full characterization of sensor properties and then perform degradation studies of poly(lactic-co-glycolic acid) (PLGA) in steady flow for three different enzyme concentrations.
This large difference clearly emphasizes the importance of determining the molar mass as well as the mass loss when performing degradation studies.
As an alternative assay, we performed cycloheximide degradation assay of RGS4, a G-protein activating protein that modulates the activity of Gα subunit.
It can achieve 61% mineralization in acidic environment, which is five times higher than that of IrO2 under the same condition and two times higher than that of performed Fenton degradation.
3) Figure 1 figure supplement 1: APC is still active in quiescent extracts based on an assay performed with degradation of securin.
A.L. performed actin-degradation experiments.
Proteasomes normally perform controlled degradation of proteins and proteins selected for degradation by tagging with a poly ubiquitin chain.
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