The synthetic wastewater for which treatment process was performed contains methyldiethanolamine (MDEA).
Hydrolysis of mannan polymers was performed containing 50 mM MES buffer (pH 6.5) and 1.0 U/mL of the recombinant β-mannanase.
Additionally, hydrolysis of 0.5% (w/v) M1 M6 was performed containing 50 mM MES buffer (pH 6.5) and 3.1 U/mL of the recombinant β-mannanase.
Hydrolysis of 0.4% (w/v) LBG, 0.2% (w/v) ivory nut, 0.2% (w/v) konjac glucomannan, or 0.3% (w/v) porang was performed containing 50 mM MES buffer (pH 6.5) and 1.0 U/mL of β-mannanase.
Then detailed ophthalmological examination were performed containing spherical refractive error, astigmatic refractive error, spherical equivalent (SE), anisometropia, best corrected visual acuity, intraocular pressure, slit lamp biomicroscopy, fundus examination, axial length, anterior chamber depth, and central corneal thickness.
Fifty µl PCRs were performed containing 5 µl diluted +1/+1 pre-amplification mixture, 75 ng HpaII primer, 75 ng MseI primer, 0.2 mM dNTP, 1 U AmpliTaq (Applied Biosystems) and 1× AmpliTaq buffer.
Twenty µl PCRs were performed containing 5 µl diluted RL mixture, 30 ng HpaII primer, 30 ng MseI primer, 0.2 mM dNTP, 0.4 U AmpliTaq® (Applied Biosystems) and 1× AmpliTaq buffer.
Polymerase chain reaction amplification was performed containing [ αP]dCTP and 50 ng of genomic DNA.
Two independent experiments were performed containing 20 and 30 mice, respectively.
A backward stepwise analysis was performed containing all the variables to identify the variables that were removed from the model.
