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To evaluate the ROBO1-binding affinity of the anti-ROBO1, we performed cell ELISA.
Then, we performed cell viability assay to determine the effects of MTUS1 on the proliferation of A549 and HBE.
To analyse the intracellular transport of PLP from the cell surface, we performed cell surface biotinylation.
To localize cellular death in the lung tissue we performed cell specific immunostaining.
We performed cell cycle analyses using propidium iodide and BrdU to label proliferating cells (Figure 1E).
BM performed cell transfection.
JS performed cell growth.
HFX performed cell culture.
CH performed cell biology experiments.
S.R performed cell synchronization experiments.
MGe performed cell sorting experiments.
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