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FDG PET/CT imaging and patient preparation was performed as outlined by Shankar et al. [22].
All experiments were performed as outlined above unless otherwise noted.
ChIP was performed as outlined by Upstate (Millipore).
The study was performed as outlined previously in detail [36], [40].
Instead of column purification phenol extraction was performed as outlined in the next paragraph.
Western blot analysis was performed as outlined [26] using a commercially available anti-his6 antibody (Sigma) and a stabilized goat anti-mouse HRP-conjugated secondary antibody (Pierce).
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Densitometric quantitation was performed as previously outlined and results were corrected to GAPDH levels, used as a housekeeper, to correct for potential errors in loading.
DNA transfections and HS were performed as previously outlined.
Complications assessments are performed as previously outlined (6), usually on an annual basis.
However, real-time PCR for A and B subunits of tox (9 ) amplified subunit A but not subunit B. Sequence analysis of the tox gene was performed as previously outlined (10 ) and compared with a reference tox gene, GenBank accession no.
The microarray analysis in this study was performed essentially as outlined in [18].
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