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Extracellular lactate measurement was performed as directed (BioVision) using a lactate assay kit kindly provided by Prof. Burlina (Department of Paediatrics, University of Padova).
Proteins were electrophoresed on 10% Tris-HCl polyacrylamide gels at 100 V for 60 min and transferred on PVDF membranes at 100 V for 60 min. Blotting was then performed as directed by the antibody manufacturer.
The hybridization procedure was performed as directed by the manufacturer.
Procedures were performed as directed by the manufacturer.
Additional laboratory testing was performed as directed by the intensivist.
The sample preparation, including DNA extraction, digestion, labelling and hybridization, was performed as directed by the manufacturer.
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I'll perform as directed but no more than that.
RHC would remain for diagnosis but would only be performed later as directed clinically.
Furthermore, in [20], they were categorized according to the level of coordination performed as direct access-based (DAB) protocols and dynamic spectrum allocation (DSA -based MAC protocols.
For these experiments, the array hybridizations were performed as direct comparisons using RNA from the two genotypes (see Table 1 and Methods).
Cases of superficial infections caused by Fusarium spp. were identified by reviewing the Mycology Laboratory database of cultures of dermatologic patients, a database that contains description of the type of lesion from which direct examination and culture were performed, as well as the results of direct exam and culture.
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