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Exact(7)
Afterwards immunochemistry was performed as below described.
All the PCR runs were performed as below: 5 min at 94°C, then 20 s at 94°C, 20 s at 61°C, and 30 s at 72°C, 30 cycles, followed by 5 min at 72°C.
Western blot analysis of HEK 293-T cells transfected with pEGFP-C1-SAG5D was performed as below: The cells were treated using RIPA Lyses Buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1% Triton X-100, 1% Sodium deoxycholate, 0.1% SDS) containing 1 mM protease inhibitor PMSF (phenylmethanesulfonyl fluoride) and centrifuged at 13,000 × g for 10 min.
The immunohistochemistry staining was performed as below.
Transfection experiments with chloroquine (Sigma-Aldrich, 100 μM) were performed as below.
Protease identification by in-solution digestion and LC-MS/MS analysis was performed as below.
Similar(53)
The coating processes were performed as described below: LFP was dispersed into methanol to form a uniform slurry by ultrasonic.
In the basal compartment, a monolayer of epithelial cells was grown and a wound scratch assay was performed as described below.
Once the possible models and their corresponding orders were found, the next step of model estimation was performed as explained below.
Cell staining was performed as described below.
Statistical analyses were performed as described below.
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