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A Taqman Low Density Array (TLDA) Human miRNA Panel (rodent panel was not available at the time the experiments were performed) (Applied Biosystems, Foster City, CA), was used for measuring the expression levels of miRNAs expressed in rat DRGs according to the manufacturer's instructions.
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This procedure was performed applying Genetic Algorithm technique.
The robustness tests were performed applying a Plackett-Burman design.
Principal component analysis (PCA) of remanent magnetization for each demagnetization data was performed applying Kirschvink (1980).
Fluorescence lifetime imaging was performed, applying the time-correlated single-photon counting (TCSPC) technique.
The syntheses were performed applying highly purified water or diethylene glycol as solvent.
The classification is performed applying the confidence-measure technique detailed in [6].
The experimental runs were performed applying a wide range of catalyst mass to molar feed ratios.
Based on this model a nonlinear control design approach is performed applying the back-stepping methodology.
The analysis was performed applying a logit transformation, forcing the response to stay between 0 and 1.
Purification of amplified or restriction fragments from gels was performed applying a GeneJET Gel Extraction Kit (Thermo Fisher Scientific Inc., Waltham, USA).
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