Exact(23)
In cases where homologous proteins (or homologous domains) were not present, we performed alignments with other nematode sequences.
We identified orthologous genes by reciprocal best match using CROSSMATCH (http://bozeman.mbt.washington. edu/phrap.docs/phrap.html) and performed alignments of BY, RM, YJM, and S. paradoxus genes and intergenic regions using CLUSTALW [48].
We performed alignments by both 5' end seed matching (nt 1 10) and by analysis of homology across complete mature miRNA sequences, comparing individual C. elegans miRNA sequences against all known C. elegans, D. melanogaster and human miRNAs, and individual D. melanogaster miRNAs against D. melanogaster and human miRNAs (Tables 1 6).
AW performed alignments, analyzed sequencing ratios, and revised the manuscript.
We also performed alignments with different number of excluded gaps.
KEB carried out the protein and domain identifications, performed alignments, phylogenetic analyses, and drafted the manuscript.
Similar(37)
We performed alignment and phylogenetic analysis separately on these repeat regions to ensure their accurate placement in the global SEP53 alignment (Figure 2, S1).
TRM, RP and MED performed alignment, transcript assembly and analysis.
To map reads with higher divergence we additionally performed alignment with BFAST [ 51].
To explore this capability, we firstly performed alignment algorithms comparison and classification.
Therefore, we performed alignment to classify H3.1, H3.3, and H3-like variants using the established signatures [ 6].
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