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To create a simple and effective method of gene knockout analysis, especially for dissecting gene functioning during insect embryogenesis, we performed a functional analysis of the Bombyx Wnt1 (BmWnt1) gene using Cas9/sgRNA-mediated gene mutagenesis.
We performed a functional analysis of regenerating muscles treated as described above to evaluate whether differential regeneration altered muscle performance.
In this study, we performed a functional analysis of a series of His-to-Ala neoculin variants to answer these questions.
Next, we performed a functional analysis of gene expression comparing the Pre-TX gene expression to each of the serial time points sampled.
Additionally, we performed a functional analysis of RECK in a breast cancer cell model.
In order to decipher the biological meaning of those opposite regulations, we performed a functional analysis of those subsets.
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In this work, we concentrate on its application in biology to perform a functional analysis of genes.
As centrality seems to be an important parameter for evolution, acting either directly or as a reporter of other underlying variables, we decided to perform a functional analysis of the most central genes.
We used Toppgene to perform a functional analysis of the list of genes up-regulated (Additional file 5: Table S4).
To perform a functional analysis of the DUSP6/ MKP- 3 promoter, we first isolated a 6.4 kb BamHI fragment containing nucleotides −6758 to −359 (where the A of the ATG start codon is +1) of the 5′ flanking sequence from the murine gene.
Apart from analyzing enrichment for GO annotations, we also performed a functional analysis based on overlap of our data with gene expression clusters in C. elegans, which originate from other published microarray data sets.
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