Iridoviruses found in sportfish have a larger genome and contain more ORFs related to immune evasion than FV3, which could also be related to improved performance of this virus on the salamander host [ 15].
The introduced mutation improved HAI performance of the VN1203 virus with chicken red blood cells (cRBC) reached the level of sensitivity attained with horse red blood cells (hRBC), but did not have any effect on the IND05 virus.
Since nonhuman primates were shown to be a suitable model for the evaluation of H5N1 influenza pathogenesis [52], next we checked the performance of the VN1203ΔNS1 virus in Rhesus macaques focusing on attenuation and immunogenicity.
Objective: To compare the performance of conventional virus isolation on cell cultures, direct detection of Ad antigens in conjunctival cells by a direct fluorescent assay (DFA) and Ad DNA detection in conjunctival swabs by polymerase chain reaction (PCR) for diagnosis of adenoviral conjunctivitis.
It is possible that some of these features influence differently the performance of a virus when it grows isolated or in competition.
The performance of the mRT-qPCR on the virus panel demonstrated neither non-specific reactions nor any inter-assay cross amplification.
Based on the results of the optimization and assesment of the performance of the primers performed using CCHF virus cDNA and PCR optimization kit (Sigma®, Germany) with different Mg++ and primer concentrations, two forward and one reverse primer were selected for routine use.
However, amplicons' performance has been very variable regardless of the virus on which they are based.
Identified patterns can be used to improve quality of service, such as the performance of anti-virus applications, for instance.
Taking into account that virus and host mRNA pools compete for the same finite translational resources in the host (s i,h), the performance of the supply chain will differ between virus (u i,v = 1 − d i,v/ s i,h) and host (u i,h = 1 − d i,h/ s i,h) perspectives as their demands differ.
