Finally, we have evaluated the performance of these transcripts as a signature in discriminating between benign tissue samples, localised PCa and metastatic disease in an additional dataset generated by Grasso et al. ROC curves reveal that the signature exceeds the performance of ERG, KLK3 or the AR as a classifier.
An unexpected observation was also that the performance of the reference transcripts were different between the platforms (data not shown).
To assess the performance of the various tools with transcript expression levels, we divide the simulated transcripts into low-, medium-, and high-expression groups based on their relative abundance A assigned by FluxSimulator (low, A<5 e−7; medium, 5 e−7< and.0001; and high, A>0.0001).
With all of these datasets, we know the truth, making them informative for evaluating the performance of transcript assembly algorithms.
On the other side of the confessional are the spray-painted words, "Cooper Union, The Musical" -- a reference to student protesters' dramatic performance of a transcript of a board of trustees meeting.
The performance of ASPic at the transcript level is quite good even at low EST coverage levels.
Suppression of the signal transduction by the pathways briefly described is consistent with biological function suggesting faithful performance of AmpliSeq-RNA transcript imaging.> -wrap-foot> The numbers refer to undetected genes in the entire panel of 917 transcripts.
As shown in Additional File 2, this analysis shows good concordance between our array and each of the other three platforms, given our selected binning, over a dynamic range of >100-fold in transcript abundance, indicating reliable performance of the new array.
Using single species RNA samples and mixed human-mouse RNA samples, we formulated and characterized two-step real-time RT-PCR assays to quantitate expression of the indicated transcripts and described analytical performance of the assays.
Much of the transcript has been redacted.
