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To test the performance of the microarray with the complex oral microbial community, we performed a spiking experiment.
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The performance of the microarray was investigated by comparison with individual ELISAs for the 12 included citrullinated peptides.
These data illustrate the high performance of the microarray analysis to identify unknown genes with potential functions in iron acquisition and new members of the Fur regulon.
Quantitative phenotyping of the complete heterozygote pool of the S. cerevisiae deletion collection was also investigated in a comparative study in which the performance of the microarray barcoding system was evaluated along with deep barcode sequencing.
Furthermore, the identity of the input populations and the performance of the microarray platforms were confirmed by correlating our mRNA expression profiles with those previously published in the differentiation map of (human) hematopoiesis (DMAP 12 (Supplementary Fig. 1d g).
This places high demands on sample preparation and technical performance of the microarray experiment.
Furthermore, the cycle performance of the microarray was excellent.
Finally, the microarray validations using semiquantitative RT-PCR corroborated the performance of the microarray providing strong support to the microarray results.
Each probe has 4 replicates within each microarray, providing technical replicates for measuring consistency and performance of the microarray.
MD assisted in the design and performance of the microarray experiments.
To further evaluate the performance of Actichip, we compared the microarray with two other well-established oligonucleotide-based platforms (Table 4).
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