Your English writing platform
Discover LudwigSuggestions(1)
Exact(6)
Computational performance (column 6) are related to a C++ implementation and run on a PC equipped with an Intel 2.4 GHz dual-core processor and 4 GB of RAM; time required for disk loading/saving of range data is excluded.
After centrifugation at 16,000 rpm for 30 min, the supernatant was loaded onto a 20-ml Phenyl Sepharose High Performance column that was equilibrated with buffer D (1 M (NH4 2SO4, 20 mM Tris-HCl/pH 7.5), and proteins were eluted with a 0 100% gradient (120 ml) of buffer A. Fractions containing the target protein, identified by SDS-PAGE, were dialyzed against buffer A overnight.
The soluble fraction was loaded onto a Ni-Sepharose high performance column (Amersham Biosciences) at 1 ml min− 1 on an ÄKTAprime (GE Healthcare).
The best separation of compounds present in the analysed polyphenol complex, was achieved by 'comprehensive' LCxLC method using Nucleodur Sphinx RP column in the first dimension and Chromolith Performance column in the second dimension.
(NH4 2SO4 was added to the protein fraction to a final concentration of 0.7 M and the mixture was applied to a phenyl sepharose (high performance) column (Amersham Biosciences) pre-equilibrated in buffer A containing 0.7 M (NH4 2SO4.
The cell suspension was sonicated five times for 30 s each and centrifuged at 15,000 rpm for 5 min. The supernatant was loaded onto a Ni-Sepharose™ high performance column (1 ml bed volume).
Similar(54)
Supercritical fluid chromatography (SFC) has recently benefited of new instrumentation, together with the availability of many ultra-high performance columns (sub −2 μm fully porous particles or sub −3 μm superficially porous particles), rendering it more attractive than ever.
The results in Table 4 suggest that training with the ambiguous failures does not impact the classification performance (columns "none" vs. "only train", and columns "only test" vs. "both").
Recombinant RaaS was purified by using a HiTrap 1-ml immobilized-metal affinity high-performance column (Amersham Biosciences).
The supernatant was incubated at 4 °C for 4 h with 500 μg of DHCR24 MoAb 2-152a immobilizedized on a HiTrap N-hydroxysuccinimide-activated high-performance column (Amersham Bioscience, Amersham, UK) according to the manufacturer's protocol.
The "Combined Performances" columns shown in Table 10 list the result of combining the performance for the T sites with the performance for the S sites based on the number of confirmed O-Glysosylated sites for each type.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com