Selenium sites were found by HYSS, experimental phases were calculated in Phaser31 and density modification with solvent flattening was performed with Resolve.
Selenium sites were identified and refined by the program SHELX [40], and density modification of the experimental map was performed with RESOLVE [46].
Currently, monolithic glass drift tubes have found use in an atmospheric pressure IM-MS instrument, where they perform with favorable resolving powers (85 t/Δ t) on scale with stacked-ring designs.
The scoreline was emphatic at the conclusion but Italy performed with great resolve.
And, despite performing with more resolve than in their 8-0 defeat at St Mary's last Saturday, Sunderland lost another bad goal in stoppage time.
Localized shimming was performed with point resolved spectroscopy [ 24] over a coronal slab (~12 × 6 × 6 mm) covering forelimb somatosensory cortex to yield a water spectral linewidth of 20~30 Hz.
Substructure solution, phasing, density modification and initial model building was performed with SOLVE and RESOLVE [48], [49] on the primitive orthorhombic crystal form.
Electrophoretic separation of proteins [ 20] was performed with discontinuous 4%stacking-10stacking-10% polyacrylamide gels in reducing conditions employing a miniVE Vertical Electrophoresis System (Amersham Biosciences Corp., USA).
Time-resolved FT-IR experiments and data analysis Step-scan spectroscopy was performed with a spectral resolution of 4.5 cm−1 and a time resolution of 5 μs.
We performed high-throughput hydroxyl radical experiments by identifying cleavage positions using primer extension performed with fluorescently labeled primers, resolved by capillary electrophoresis [30], [31], [32].
All western blots were performed with whole cell lysates, resolved by SDS-PAGE, transferred to PVDF (Immobilon-P, Millipore, Billerica, MA), and blotted for SOCS-1.
