Your English writing platform
Discover LudwigExact(1)
We used FASTX-Toolkit to perform trimming, quality filtering, and duplication removal [ 37].
Similar(59)
Those few not performing trimming reported it was either not necessary for their intended use, performed automatically by their NGS software or will consider trimming in future analyses.
This differentiation in the magnitude of concordance improvement, along with the fact that performing trimming leads to a more significant improvement in reads' quality for S2 data set, further indicates that Novoalign is more sensitive towards the changes in sequencing quality.
The basic idea of a mode-finder is first to identify a central value of either luminance or chromaticity, termed 'mode' across all the observations at every pixel then perform trimmed LS only using the observations that are with a certain range around the mode.
Comparison results from applying both filterY and trim steps (filterY&trim) with those from performing trim step alone also revealed the useless of filterY step on improving SNP calling performance (Table 2 and Figure 1E).
To improve the quality of the data fastx_trimmer (see Code availability 2) was used to perform hard trimming of the sequenced reads (note: when performing hard trimming on HiSeq data, rather than GAII data, an additional –Q 33 switch was needed in the command).
[Editors' note: the author responses to the re-review follow.] 1) Authors were asked to perform the trimming assay with mutanolysin.
The manuscript is much improved but there are some remaining issues that need to be addressed before acceptance. 1) Authors were asked to perform the trimming assay with mutanolysin.
We perform quality trimming by removing all positions including and to the right of the first position that has a quality score of less than 15, resulting in a size of 1.3 G for the mouse libraries, 2.5 G for the rat libraries and 1.1 G for the human libraries.
The selection of the recorded ROI was performed by trimming small square regions (around 200 µm in width) with a beveled edge diamond knife (cryotrim, Diatome).
SPF selected dense array of BACs for end sequencing and performed sequence trimming.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com