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In the first work a Tantalus-like figure reaches for an elusive pitcher of water that dangles from the heavens; in the second two men of notably different metabolisms perform the empty rituals of daily life.
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Resequencing function is performed with the empty locations in the buffer.
These simulations were performed with the empty and with the ADP- and ATP-loaded AAC as well as with the poorly transported AMP and guanine nucleotides, showing in the free energy landscapes that ADP and ATP lowered the activation free-energy barriers more than the other substrates.
Control experiments were performed with the empty pcDNA vector.
Controls were also performed with the empty miniCTX2 integrated into each mutant strain.
The IMR32 nuclear extracts and the in vitro expressed PHOX2B-Myc fusion protein were prepared as previously described [17]; the in vitro reaction was also performed with the empty vector (pcDNA3.1 M/H) to be used as negative control in the EMSAs.
The 4-day cytotoxicity assays were performed on the empty vector transfected cells as well as the 482G-1, 482R-2, and 482T-10 transfecloneslones.
The negative controls, performed with the empty p406- CYC1 plasmid and with the DNA that was isolated from the empty yeast host, did not show the reporter-specific 873-bp band.
The negative controls, performed with the empty p406- CYC1 plasmid and with the DNA that was isolated from the empty yeast host (the nontransfected yeast cell), showed no PCR bands.
Quick Change Site Directed Mutagenesis was performed with the empty pCMV vector to introduce a SacII site 5′ to the MluI site, followed by digestion of the vector with SacII/ MluI and introduction of flLRRK2.
Titania's images were apt when the play was first performed – "The fold stands empty in the drowned field … The nine men's morris is filled up with mud" – and are no less apt today.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com