Sentence examples for perform tandem from inspiring English sources
The tagged proteins can be used to perform tandem affinity purification (TAP) or chromatin immunoprecipitation coupled with microarray analysis (ChIP-CHIP).
Postural control activities and balance training were also provided for 10 min by asking participants to perform tandem gaits and one leg standing with eyes open/close (up to ones' ability), step up and down stairs, toe walking and heel walking.
We explored the T. gondii proteome by performing tandem mass spectrometry (MS/MS) experiments on T. gondii plasma membrane, cytoskeletal and cytosolic protein preparations.
To prepare the minichromosomes for AFM and the remodeling assays, we performed tandem purification through successive linear 15 30% sucrose gradient and 30% sucrose cushion ultracentrifugation.
In order to have a wide picture of the set of membrane proteins interacting with Bcl-xL, we performed Tandem Affinity Purification from total cellular membranes of HeLa cells stably expressing TAP-tagged Bcl-xL.
To confirm the identity of the abnormal protein bands and prove that these represent genuine examples of the expected mutant proteins, we initially performed tandem mass spectrometry on immunoprecipitates of the cell lines.
With the aim of identifying the PcG proteins that functionally associate with CBX7, we performed tandem affinity purification of complexes based on mouse Cbx7 (mCbecauseecause of its relative stability, as well as human BMI1 and the closely related Psc protein MEL18.
By performing tandem MS, we were able to isolate and activate each of the different tetramers.
We previously performed tandem affinity purification (TAP) of protein complexes from Tiam1−/− mouse embryonic fibroblasts expressing TAP-tagged TIAM1 (Mack et al., 2012).
We next performed tandem affinity purification, although yields were not high because of the low levels of tagged Oct4 and purification efficiency.
To confirm the Mad2p phosphorylation sites in vivo, we performed tandem affinity purification of Mad2-His-TEV-Biotin (HTB) from both cycling and nda3 arrested cells.
